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Robert 2nd May 2011 07:10 PM

Re: TC question
 
Quote:

Originally Posted by winterkid (Post 47380)
robert..
what kind of medium you use??
i saw that its a little blue and translucent..
mind sharing the recepie with me?*biggrin2*
and where did you get the TC vessels?

the containers/vessels were bought from a local scientific co.

i made my own medium - sugar, coconut water, charcoal and agar.

winterkid 2nd May 2011 07:54 PM

Re: TC question
 
mind sharing the location or contact of the scientific co?

can share the exact perpotion of the ingredient for the recepi

Robert 2nd May 2011 08:46 PM

Re: TC question
 
Quote:

Originally Posted by winterkid (Post 47389)
mind sharing the location or contact of the scientific co?

can share the exact perpotion of the ingredient for the recepi

I'm in kuching and the co. imported the jars ( and other equipment and accessories ) were imported from, most likely, kuala lumpur.

To a liter coconut water ( or a liter distill water ) i add sugar 20 gm, charcoal 0.5gm, agar 8 gm ( the degree of softnes and watery-like to hardness of the medium depended on the amount of agar, you may play with the amount) 8gm is still soft. Too hard the seedlings roots can't penetrate the medium.

I read and gathered infos from the following link and try it out my own. you may like to prepare your own.
http://www.orchidboard.com/community...ng-medium.html

There is no right or wrong answer. some people add apple juice, banana, papaya, tomato. The ingredients are important to seedlings development else without food the seedlings turn brown and die.

Harris Azariel 2nd May 2011 10:15 PM

Re: TC question
 
whats charcoal for?

so you use the spirit metil(the once in bengkil) as for sterilization?

Robert 2nd May 2011 11:43 PM

Re: TC question
 
Quote:

Originally Posted by Harris Azariel (Post 47394)
whats charcoal for?

so you use the spirit metil(the once in bengkil) as for sterilization?

role of charcoal
http://www.ncbi.nlm.nih.gov/pubmed/18786626

for sterilisation i use ethanol, but preferable household bleach(clorox).

Harris Azariel 2nd May 2011 11:51 PM

Re: TC question
 
is ethanol rubbing alchohol?
hw much does it cost for hw much ethanol?

Harris Azariel 2nd May 2011 11:53 PM

Re: TC question
 
so u take the dust of the coal and measure till 0.5grams?


Thanks man!
Harris

Robert 3rd May 2011 02:05 PM

Re: TC question
 
Quote:

Originally Posted by Harris Azariel (Post 47397)
is ethanol rubbing alchohol?
hw much does it cost for hw much ethanol?

rubbing alcohol is methanol, contain ethanol but not as pure. yes, can be used to sterilised seeds and the working area ( inner sides of the glove hood and laminar floor)

ethanol was packed in 2.5 lit container.

You need to find the price in KL or anywhere in west malaysia, 'cos we are paying more for most item in the east.

Robert 3rd May 2011 02:06 PM

Re: TC question
 
Quote:

Originally Posted by Harris Azariel (Post 47398)
so u take the dust of the coal and measure till 0.5grams?


Thanks man!
Harris

i crush the charcoal then sieve and use the finer parts.

Harris Azariel 3rd May 2011 02:36 PM

Re: TC question
 
Rob,can u sure us the pic of the bottle of the ethanol?


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