Tissue Culture Thread
 

Hi

just want to show some jars from me.

Here a VFT from Seed (5month old)
http://pics.nepenthes.se/forumpics/11.08/img_0655.jpg

VFT Red form. TC from Leaf cutting, Now develop their first leaves after i replant this to plain media.
http://pics.nepenthes.se/forumpics/11.08/img_0657.jpg

VFT B52 make now callus. i just transferred it to plain media and hope there come the first leaves soon
http://pics.nepenthes.se/forumpics/11.08/img_0658.jpg

here some other VFT from seeds
http://pics.nepenthes.se/forumpics/11.08/img_0660.jpg

http://pics.nepenthes.se/forumpics/11.08/img_0662.jpg

Jens

Re: Tissue Culture Thread
 

Hi Nepaholic

Nice VFts I like them.........You grew the seeds inside the syrup thingy????????????

Ken

Re: Tissue Culture Thread
 

Nice VFTs. How long did it take for them to germinate??

Re: Tissue Culture Thread
 

Thank you

it takes 4-6 weeks for germination in TC.
But then they grow very fast.
The last 2 seedlings i sow in august. So they germinate in September.

You can see how fast they grow, every day

Jens

Re: VFT TC Pictures
 

may we know what TC medium you use and what preparations you made in terms of chemicals, etc. on your seed and on your leaf cutting? Did you use hormones, etc?

I'm hoping that I can finally buy a TC kit and start a project similar to yours.

Oh and where do you keep the bottles? What lighting?

TTFN
Arvin

Re: VFT TC Pictures
 

Hi Leafs are little bit tricky and i recommend to learn first TC with seeds.
I use 1/4 ms for seeds, for leafs just add 1ml/L Bap.
Seeds i sterilise 15min in 10%Bleach
Why you want to buy a kit?
You can build a simple transfer box with gloves. thats cheaper.

Jens

Re: VFT TC Pictures
 

Hi Jens...

How are you doing bro? it has been so long since we last chatted...

Nice Tc plant... good job... :1thumbup::1thumbup::1thumbup:

Later i might wanna get some info from you^^

Regards
Jonathan

Re: VFT TC Pictures
 

Hi Jonathan

everything its ok. Just a little bit busy :)
How are you?

Re: VFT TC Pictures
 

Can it be done with any CP seeds??

Re: VFT TC Pictures
 

BTW what's that syrup-thingy.

Re: VFT TC Pictures
 

Yeah, whats that liquid, I am interested. Perhaps an alternative to a different media finally!

Cheers

Re: VFT TC Pictures
 

here are some links for all who didn´t know what TC is
http://en.wikipedia.org/wiki/Growth_medium

Re: VFT TC Pictures
 

Re: VFT TC Pictures
 

Ooh.....what language is that??

Re: VFT TC Pictures
 

Swedish

Re: VFT TC Pictures
 

Jonathan, thanks for your reply about what medium, etc. you use. appreciate it. Actually I was going to buy a TC kit that includes agar, MS, etc. it does not come with a laminar flow box, etc. Which now you mentioned it, do you do your TC in your "kitchen" meaning you made your own box? May I request for a photo of your setup? :)

To the others who were asking for info about the medium and didn't visit the link, basically that syrupy thing is agar medium, it is not really syrupy because it is like jello, heck one can say it's jello. I have some Gelatine mix now because I wanted to experiment, my first experiment failed, the Jello never stayed hard in room temperature. Also you can't technically use it for outdoor or normal potting, because the medium, if you enrich with nutrients for the plants, can help mold, bacteria, etc. grow quickly, thus if your medium gets contaminated with mold for example, your plant will be compromized. I am still new to this so I hope what I said was accurate, anyone please jump in if any of what I said is incorrect. :)

TTFN
Arvin

Re: VFT TC Pictures
 

hi

I build a laminar flow hood self. Very expensive things but veryg good.
Since i have this, i had no contaminations.
Here is my flowhood:
http://pics.nepenthes.se/forumpics/07.08/img_8896.jpg

http://pics.nepenthes.se/forumpics/07.08/img_8895.jpg

http://pics.nepenthes.se/forumpics/07.08/img_8901.jpg

http://pics.nepenthes.se/forumpics/07.08/img_8905.jpg

The candle is just for checking the flow
http://pics.nepenthes.se/forumpics/07.08/img_8909.jpg

Re: VFT TC Pictures
 

Jens, That's impressive workmanship. Nicely done!

Re: VFT TC Pictures
 

Ooh how confusing.

Re: VFT TC Pictures
 

Hi Arvin...

I guess you got the wrong guy... you should be thanking Jens... :1thumbup:

Hej Bro...

looks very good and very impressive.... hope i could get the the blue print of the laminar floor hood from you...

Regards
Jonathan

Re: VFT TC Pictures
 

Yes my apologies, Thanks Jens, and thanks for sharing your Laminar flow system. May I know if there are any free plans for these in the internet, or maybe a way for you to share your design?

Your system looks really nice! There are some TC labs in universities here that use a more simple system of a closed box with glass and working with gloves, where you put your hands in the gloves from outside so that the box is "sealed", the problem I think is it is difficult to still prevent contaminants from coming in when you open the box to put in materials and instruments.

TTFN
Arvin

Re: VFT TC Pictures
 

Thanks Guys

here it the plan i build my flowhood after. They are nearly same. i just bought an other blower. but my blower have nearly same power.

http://www.orchideenvermehrung.at/english/lfh/index.htm

Re: VFT TC Pictures
 

Ooohhhh......what is a laminar flow system? It looks well-built, I wonder, does it control the flow of air??:confused:

John, I didn't know you could write swedish. Cool!

Yes, that was certainly educational, I think that will be a important ingredient in the CP world one day.....

Cheers

Re: VFT TC Pictures
 

Ooh...I hope I can germinate darlingtonia in TC jello. That will make them grow faster.

Re: VFT TC Pictures
 

So,how did you get the jelly thingy?(still didn't get the name.........zzzzzzzz)

Re: VFT TC Pictures
 

TC Jelly?

Re: VFT TC Pictures
 

the gelling agent is agar, mixed with MS medium and some other things

Jens

Re: VFT TC Pictures
 

Does nurseries sell them?

Re: VFT TC Pictures
 

nursery didn't sell them, you can buy it online or see if someone you know is doing tissue culture lab.

Re: VFT TC Pictures
 

i want to ask about the system, did you use UV light to sterilize the compartment before you started to use it?

Re: VFT TC Pictures
 

No i just clean the bench with alcohol before i start work in it.
I can have jars open for 30min without any contaminations.

Jens

Re: VFT TC Pictures
 

Here some new Pictures. Sorry for the poor quality, It is not easy to take photos of plants inside jars

VFT Giant
http://pics.nepenthes.se/forumpics/12.08/img_0952.jpg

B52 now develop the first leafes
http://pics.nepenthes.se/forumpics/12.08/img_0953.jpg

Drosera peltata
http://pics.nepenthes.se/forumpics/12.08/img_0955.jpg

Red VFT
http://pics.nepenthes.se/forumpics/12.08/img_0956.jpg

http://pics.nepenthes.se/forumpics/12.08/img_0957.jpg

http://pics.nepenthes.se/forumpics/12.08/img_0959.jpg

Red from Leaf Tissue
http://pics.nepenthes.se/forumpics/12.08/img_0961.jpg

D. auriculata
http://pics.nepenthes.se/forumpics/12.08/img_0963.jpg

Jens

Re: VFT TC Pictures
 

Nice plants. I find out that the seed cultured germinated faster.

Cheers

Re: VFT TC Pictures
 

Hey I actually agree, I wonder why not many TC labs use UV light to sterlize the case before working, that should technically work well! I do like Jen's system, very simple yet he is producing nice TC plants.

By the way when you posted VFT red from leaf tissue, does that mean the leaf or the white part of the leaf?

As usual great work and informative! Need to get going and order my kit soon :)

TTFN
Arvin

Re: VFT TC Pictures
 

Hi
First it is expensive. And Alcohol work faster. If you want to sterilize with UV lights it needs severall hours to be clean. Alcohol works directly.

Why simple? It is a flowhood like the big labs also use, just cheaper because i build it self.
I tryd many different ways to do TC but this is really the best.
I tried over a cooking pot, gloveboxes....

Gloveboxes are good if you want to start with TC and it is also cheap to build.

I used the white part of the leaf.
Here you can see a picture of the same plant, just 1 month ago
http://pics.nepenthes.se/forumpics/11.08/img_0657.jpg

@edwardyeeks
you really think they are faster?
I think not because VFT seeds germinate 2-3 later as usual. Also Nepenthes and Drosera
Tc needs some time but then after a while the seeds/plants whatever kick off :)

Jens