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-   -   Tissue Culture Thread (https://forum.petpitcher.net/showthread.php?t=1513)

nepaholic 11th November 2008 07:56 PM

Tissue Culture Thread
 
Hi

just want to show some jars from me.

Here a VFT from Seed (5month old)
http://pics.nepenthes.se/forumpics/11.08/img_0655.jpg

VFT Red form. TC from Leaf cutting, Now develop their first leaves after i replant this to plain media.
http://pics.nepenthes.se/forumpics/11.08/img_0657.jpg

VFT B52 make now callus. i just transferred it to plain media and hope there come the first leaves soon
http://pics.nepenthes.se/forumpics/11.08/img_0658.jpg

here some other VFT from seeds
http://pics.nepenthes.se/forumpics/11.08/img_0660.jpg

http://pics.nepenthes.se/forumpics/11.08/img_0662.jpg

Jens

kentosaurs 11th November 2008 11:38 PM

Re: Tissue Culture Thread
 
Hi Nepaholic

Nice VFts I like them.........You grew the seeds inside the syrup thingy????????????

Ken

plantlover 12th November 2008 12:11 AM

Re: Tissue Culture Thread
 
Nice VFTs. How long did it take for them to germinate??

nepaholic 12th November 2008 12:31 AM

Re: Tissue Culture Thread
 
Thank you

it takes 4-6 weeks for germination in TC.
But then they grow very fast.
The last 2 seedlings i sow in august. So they germinate in September.

You can see how fast they grow, every day

Jens

arvin555 12th November 2008 01:37 AM

Re: VFT TC Pictures
 
may we know what TC medium you use and what preparations you made in terms of chemicals, etc. on your seed and on your leaf cutting? Did you use hormones, etc?

I'm hoping that I can finally buy a TC kit and start a project similar to yours.

Oh and where do you keep the bottles? What lighting?

TTFN
Arvin

nepaholic 12th November 2008 07:14 PM

Re: VFT TC Pictures
 
Hi Leafs are little bit tricky and i recommend to learn first TC with seeds.
I use 1/4 ms for seeds, for leafs just add 1ml/L Bap.
Seeds i sterilise 15min in 10%Bleach
Why you want to buy a kit?
You can build a simple transfer box with gloves. thats cheaper.

Jens

Jonathan 12th November 2008 07:35 PM

Re: VFT TC Pictures
 
Hi Jens...

How are you doing bro? it has been so long since we last chatted...

Nice Tc plant... good job... :1thumbup::1thumbup::1thumbup:

Later i might wanna get some info from you^^

Regards
Jonathan

nepaholic 12th November 2008 08:06 PM

Re: VFT TC Pictures
 
Hi Jonathan

everything its ok. Just a little bit busy :)
How are you?

plantlover 12th November 2008 09:03 PM

Re: VFT TC Pictures
 
Can it be done with any CP seeds??

plantlover 12th November 2008 09:03 PM

Re: VFT TC Pictures
 
BTW what's that syrup-thingy.


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