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Old 8th November 2009, 12:58 PM
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jason840220 jason840220 is offline
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Location: Subang Jaya, Selangor
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Default Re: tissue culture

As far as I am concern, leafs micropropagating nepenthes or sarracenia are fairly difficult. These plants develop such symbiosis relationship with the microbs contains inside their traps. In such, it's hard to get 'em sterilized. Perhaps TC using seeds is the best option. One of our forum member had tried but to no avail. Nevertheless, I never tried it on pitcher plants though.

I don't know 'bout the chemical contain in your kit. But it should come with phytohormones and PPM (or at least equivalent). The ratio of auxin to cytokinin in the media determines initiation of root versus shoot buds. Hence TC'ng using ready made kit yield higher success rate. Often, home made TC set struggle to have plantets to root.
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