Read-Only Forum Archive
View Single Post
  #13  
Old 17th June 2009, 09:18 AM
paphioboy paphioboy is offline
Global Moderator
 
Join Date: Tue Dec 2008
Location: Penang
Posts: 672
Default Re: Nepenthes Tissue Culture

Quote:
I was also asking about the practicality or sucess rate with using Apical Meristem from an adult nepenthes cutting, because so far I have read that usually cuttings are already contaminated by a lot of organisms that it is almost impossible to get a sterile sample to be able to do TC.
The apical meristem used should be relatively clean compared to the rest of the plant because it is hidden beneath all the other young leaves. If unsure of contamination, can soak in 5% bleach solution to surface-sterilise it before TC.
Reply With Quote