[paphioboy]

Tissue culture is not easy to DIY for the average CP grower. To be successful, you must have a completely sterile environment. The reason is that agar is nutrient rich, so even a tiny speck of fungus/bacteria will contaminate the entire container/flask and kill all the seedlings inside. Most commercial labs have at least an autoclave, laminar flow hood with air filter and a shaker. It is much easier to send a piece of tissue to the lab and ask if they will culture it for you for a small fee.

Arvin, the apical meristem does not come from the callus. The apical meristem is taken from the adult plant. When chopped up and under the influence of hormones, it then produces the callus, which grow into many individual plantlets.