Re: Nepenthes Tissue Culture
Thanks Paphioboy for replying. first of all regarding IBA, I received IBA with my TC kit, that is why I asked about the IBA. So it is okay to use IBA even if I was going to stick the cutting in potting mix? I'm just afraid that it might promote fungal or bacterial growth. I need to read up why IBA was included in my TC kit though.
As for my other question, yes sorry I didn't realize it's two questions in one... but you are correct however that I was asking about both. However....
1. I understand that for Nepenthes, what they do is to germinate seeds in a sterile environment (Agar) so that the plantlets will be sterile, then they use those to start Tissue culture. Which now I imagine is the same as what you described with the callus. I just don't know how one can get Apical Meristem from those small plantlets, unless they just chop the whole planlet up and do as what you described?
2. I was also asking about the practicality or sucess rate with using Apical Meristem from an adult nepenthes cutting, because so far I have read that usually cuttings are already contaminated by a lot of organisms that it is almost impossible to get a sterile sample to be able to do TC.
TTFN
Arvin
|