Re: in vitro first sprout
 

orchid in vitro propagation-my successful trial with hand pollination of cross between phalaenopsis bellina x phalaenopsis cornu cervi. It took about 9 months for the seed pods to ripe but uncrack. I only need to sterilise the outer skin by dipping in ethanol, and flame over the burner.

formation of protocorms
http://i191.photobucket.com/albums/z...u/c9c80c75.jpg

2 months later after replating to several jars.
http://i191.photobucket.com/albums/z...u/b6698daf.jpg

Re: in vitro first sprout
 

Congrats on your achievement. I cant even get my phalaenopsis to flower, cnt provide the low temp drop. Any tips?

Re: in vitro first sprout
 

how do you grow your phal ... do you grow them in pot, what media do you use, or they are mounted on wood/fern bark?), they are grown under nursery shade?

Re: in vitro first sprout
 

Grow in pot and under shade. Media i experiment with all charcoal and also all sphag moss, both also the same for me.

Re: in vitro first sprout
 

why not try mounting on wood or fern bark for a change. I have the same problem in the past until i mount them on fern bark. I also fertilise them with mild orchid fertilizer once a week. Light water misting every morning and evening.