in vitro first sprout

[Robert]

sharing with everyone my low tech set up and my first in vitro neps seeds germination. seeds sterilisation was using clorox and media was home made.

contamination rate was very high for me. There are several reason - from the glass hood ( fish aquarium ) construction method, sterilisation and handlings.



jars and containers were placed on window edge where it was bright and warm



still no germination



the first sprout among all the jar

[allenphoon]

can share the advantage using the vitro? i only know that it will grow much faster
BTW, i like this picture, look like a laboratory lab

[cbkhoon]

wow.. going to next level.. good luck on your new project !

[Harris Azariel]

where did u find Agar?

[rhealk]

Wow, nice setup!

[Robert]

yeah allen it was a mini lab. There are many reason if ones searched, as an example :
http://www2.ulg.ac.be/cedevit/english/advantage_e.htm

Originally Posted by cbkhoon

wow.. going to next level.. good luck on your new project !

hee..hee not that fast. I'm still a new hand in this field. Just curious and to try out the media preparation and sterilization method.

Harris the agar² can be bought from any grocery stores or where those specialised in selling ingredients for cakes making.

Thanks rhealk

[lcuclcu]

Originally Posted by Robert

sharing with everyone my low tech set up and my first in vitro neps seeds germination. seeds sterilisation was using clorox and media was home made.

contamination rate was very high for me. There are several reason - from the glass hood ( fish aquarium ) construction method, sterilisation and handlings.



jars and containers were placed on window edge where it was bright and warm



still no germination



the first sprout among all the jar

a great project....your tools are very nice

[allenphoon]

if i really can spare vft seeds, will you try?

[arvin555]

Hi Robert,

From what I understand, PPM is an additive to the Agar itself, and prevents contamination on the medium... contamination of your Tissue is of course through sterilization such as your mentioned Chlorine, some actually say Hydrogen Peroxide, etc. I also think that once the Tissue is in the TC medium, the PPM will also probably protect it from Contamination as well.

I have a feeling that Kitchen Tissue Culture will be able to ship directly to you, if you order from them. That would already be as simple or as complicated as buying from Peninsular Malaysia.

Just my 2 cents here, I think that because VFT leaves are easy to disinfect... no need to start from seeds, you can just cut a leaf or two and that is the start of your Tissue, just disinfect the leaves or pieces properly. At least this way, no waiting for germination. As mentioned the only CP that is difficult to TC are Neps, because apparently getting Tissues from nepenthes plants and successfully and effectively sterilizing them is almost impossible.

So if you don't mind, and have the time.... May I challenge you or request you to please experiment by cutting up one of your more common nepenthes plant (just a stem cutting don't worry) and try to get a Meristem from it and then try to get that going in TC medium.

TTFN
Arvin

[Robert]

Arvin, i've read from many who had tried, and agreed neps was impossible to TC using their stems. Infact non of their shoot tip, leaf, buds, stem or root tissue can be TC.