Re: in vitro first sprout
 

u can make them root fast by putting a few of the rooting hormone that u can obtain at local store.not just fast,but the roots will be crowded with each to other strands :)

Re: in vitro first sprout
 

Hi Arvin, i've read in most post ppm was highly recommended. I have problem getting most chemical locally. Suppliers are not keen for small purchase. In most cases it had to be flown from the peninsular, and costly. Thus i use clorox and spirit which turn out as good, and inexpensive. The only problem was the wide margin of error. contamination,i read , can happen due to the age of spirit and clorox. using these to sterilise seeds ones have to adjust the time to sterilise seeds.

thanks Harris, if you have some to spare*biggrin2*

the detail from my pmed, you need to call or email them for the items you required.

Re: in vitro first sprout
 

a great project....your tools are very nice

Re: in vitro first sprout
 

if i really can spare vft seeds, will you try?

Re: in vitro first sprout
 

Hi Robert,

From what I understand, PPM is an additive to the Agar itself, and prevents contamination on the medium... contamination of your Tissue is of course through sterilization such as your mentioned Chlorine, some actually say Hydrogen Peroxide, etc. I also think that once the Tissue is in the TC medium, the PPM will also probably protect it from Contamination as well.

I have a feeling that Kitchen Tissue Culture will be able to ship directly to you, if you order from them. That would already be as simple or as complicated as buying from Peninsular Malaysia.

Just my 2 cents here, I think that because VFT leaves are easy to disinfect... no need to start from seeds, you can just cut a leaf or two and that is the start of your Tissue, just disinfect the leaves or pieces properly. At least this way, no waiting for germination. As mentioned the only CP that is difficult to TC are Neps, because apparently getting Tissues from nepenthes plants and successfully and effectively sterilizing them is almost impossible.

So if you don't mind, and have the time.... May I challenge you or request you to please experiment by cutting up one of your more common nepenthes plant (just a stem cutting don't worry) and try to get a Meristem from it and then try to get that going in TC medium.

TTFN
Arvin

Re: in vitro first sprout
 

Re: in vitro first sprout
 

Thanks allen someone had sent me sarras and vft seeds.

Re: in vitro first sprout
 

if u have success on VFTs and Sarrs,u can sell them!

Re: in vitro first sprout
 

Robert, Aww oh come on! Try Meristem! Cut a piece of nepenthe stem, then sterilize... Then cut off a few layers and then sterilize, until you get to the middle part of the stem (meristem) then sterilize and then TC :) I heard that they said that the hard part is to get a sterile sample... I don't know if that is accurate, I didn't hear anyone say that the tissue does not work, it's that usually you usually find that the Tissue gets contaminated. But as I said that is based on what I heard or read. I might have had some discussion about it here somewhere.

In any case for nepenthes, the norm is to TC the plants that sprout from seeds, at least those are sterile.

TTFN
Arvin

Re: in vitro first sprout
 

maybe one day when i've developed the skilled with all the sophisticated chemicals available i would give a try.