TC question
 

i would like to have someone teach me more about tc ....
i just wan to know the mixture for their medium and where can i get the ingerdients....?
pls discribe the ingerdients more........because i may not understand....... http://www.flytrapcare.com/phpBB3/im...on_redface.gif
i saw the web and found
http://www.phytotechlab.com/Detail.aspx?ID=1016
its salt for neps but can it be used for vfts?
i really need step by step guide on TC medium http://www.flytrapcare.com/phpBB3/im...con_e_wink.gif*biggrin2*
where do i get MS?????

Re: TC question
 

I have no experience in tc.
You can check with Quicklab in Batu Lanchang. I used to buy reagent grade chemical salts there. Mixing MS from component salts is tricky. Probably they have MS. I'll PM you their contact.

Re: TC question
 

Tan,PM me their contact info also!!

thanks!!

Re: TC question
 

what about Tc vessels...and sterlizing equepments???? where to find?*biggrin2*

Re: TC question
 

I'll be honest with you guys, I've heard that TC is quite hard to do. Even setting up TC materials can be troublesome and kinda expensive. A few members here do it, but most don't because it requires a depth of knowledge to actually do TC. But it's no harm done if you guys are willing to try. Good luck! :smile:

Re: TC question
 

From what i know, if you really willing to start TC, you will have to bear some problems

1)scolded by your mom or your wife(you need to use pressure cooker-to kill germ, etc)
2)pay lots of electrical bills( you will need artificial light instead of natural life as they are keep in vessel)
3)space(you will have to set up a completely no germ/microorganism condition for tc preparation/separation)
4)distilled water(just as the ingredient)

If you are a student, it is not advisable as it cost a lot and require long patience

Re: TC question
 

haha yes that might happen.

But i surely wont be trying it(if i want to try) now since im now very busy with studying for my PMR soon.

Re: TC question
 

The cheapest startup cost should be a few hundreds. Not too useful for small scale grower. More for learnings I believe.

Bear in mind some chemical substances are hazardous. I wouldn't recommend those haven't been trained in chemistry (till around STPM) to play with it without guidance.

Re: TC question
 

agreed. Scolding by my mom cant be avoided.....there is a hall that is ideal for a 0bacthria area because there is not much air flow......building a lab wil b my after pmr project.....

Re: TC question
 

And I am going to start a praying mantis terrarium this comming school holiday. . . .
Any species to recommend? And where do I get the mantis?

Re: TC question
 

How to do TC ...there are tons of info in the web. print out those you find useful and read them like the holy book:laugh:or jot down the important info.

The mixture - for a beginner to try out, there is no need to use expensive ingredients. Home made medium will do, the same preparation as orchid growers do.what is required, read the following link.

http://www.rv-orchidworks.com/orchid...opagation.html

To sterilise jars(with or without ingredients) and water ordinary pressure cooker is required.

jam jars, beer mugs are excellent. ordinary plastic containers can be used but need to be sterilised before pouring in the cooked ingredients. This has to be done in a sterile chamber ( a laminar flow or glove hood)

different jars that i use
http://i191.photobucket.com/albums/z...u/2f4657fe.jpg


germination in-vitro. nepenthes are difficult and near impossible to TC from tips, stems, roots or leaves. start with seeds first.

http://i191.photobucket.com/albums/z...u/046099d4.jpg

http://i191.photobucket.com/albums/z...u/04be5f5b.jpg

...and i use artificial light placing the jars on window sills, lights are filtered.
http://i191.photobucket.com/albums/z...u/414983cb.jpg

MS and other mixtures,equipments and accessories can be bought from most scientific co.

I use fish aquarium as sterile hood.
https://forum.petpitcher.net/showthread.php?t=4471

Re: TC question
 

Wow Robert, that's very nice and complex! How long did it take for the seeds to germinate?

Re: TC question
 

Thanks Robert!

For me now,keeping the media sterile when its out from the microwave is hard since that i cant tight the cap and most of my media contaminated at that part.

Re: TC question
 

22 days.

Re: TC question
 

for the sterlie hood......how do you put or remove the TC from it without contaminating them?? mind showing pics or state steps??*biggrin2*

Re: TC question
 

heres how,
http://www.youtube.com/watch?v=wJeEDKYfeoA

Frank only use normal open wide hoods but he had lots of succesfull TC vessels.

Re: TC question
 

i found this web page...
and its going to open in malaysia....
http://www.caissonlabs.com/services.php?group=2

*biggrin2**biggrin2*woopie

Re: TC question
 

before carrying out work the inside hood have to be sterilised using bleach or spirit. The jars with the ingredients are cooked withr pressure cooker for 25 minutes and they are sterile. They are opened only inside the sterilised hood ( do not open outside as that will let contaminants in )

Re: TC question
 

hmmm, the polycarbonate vessels look exactly i'm using:smile:

Re: TC question
 

robert..
what kind of medium you use??
i saw that its a little blue and translucent..
mind sharing the recepie with me?*biggrin2*
and where did you get the TC vessels?

Re: TC question
 

the containers/vessels were bought from a local scientific co.

i made my own medium - sugar, coconut water, charcoal and agar.

Re: TC question
 

mind sharing the location or contact of the scientific co?

can share the exact perpotion of the ingredient for the recepi

Re: TC question
 

I'm in kuching and the co. imported the jars ( and other equipment and accessories ) were imported from, most likely, kuala lumpur.

To a liter coconut water ( or a liter distill water ) i add sugar 20 gm, charcoal 0.5gm, agar 8 gm ( the degree of softnes and watery-like to hardness of the medium depended on the amount of agar, you may play with the amount) 8gm is still soft. Too hard the seedlings roots can't penetrate the medium.

I read and gathered infos from the following link and try it out my own. you may like to prepare your own.
http://www.orchidboard.com/community...ng-medium.html

There is no right or wrong answer. some people add apple juice, banana, papaya, tomato. The ingredients are important to seedlings development else without food the seedlings turn brown and die.

Re: TC question
 

whats charcoal for?

so you use the spirit metil(the once in bengkil) as for sterilization?

Re: TC question
 

role of charcoal
http://www.ncbi.nlm.nih.gov/pubmed/18786626

for sterilisation i use ethanol, but preferable household bleach(clorox).

Re: TC question
 

is ethanol rubbing alchohol?
hw much does it cost for hw much ethanol?

Re: TC question
 

so u take the dust of the coal and measure till 0.5grams?


Thanks man!
Harris

Re: TC question
 

rubbing alcohol is methanol, contain ethanol but not as pure. yes, can be used to sterilised seeds and the working area ( inner sides of the glove hood and laminar floor)

ethanol was packed in 2.5 lit container.

You need to find the price in KL or anywhere in west malaysia, 'cos we are paying more for most item in the east.

Re: TC question
 

i crush the charcoal then sieve and use the finer parts.

Re: TC question
 

Rob,can u sure us the pic of the bottle of the ethanol?

Re: TC question
 

Harris, try google or through ebay. They are sold in different quantity in various brand.

Re: TC question
 

Hi Harris..if you are using the original cap, dont tighten it when they are put into the oven. cap it loosely. After sterilisation wait for them to cool down, tighten the caps as u take them out.