tissue culture

a great hellow to fellow CPers.

i recently got interested in propagating CPs through tissue culture.. there seems to be so much agar recipes around that i dont know which one to use for CP propagation..

anyone had any experience with this?

[arvin555]

http://www.kitchenculturekit.com/Catalog.htm

Check out the link they have a CP special which uses MS for their recipe, murashige and skoog

I too am interested but haven't started yet at all!

TTFN
Arvin

[edmund83]

ya... i am interest too.. but i need an environment to do this....

[Aliamyz]

Good luck to everyone trying this but remember you'll need a sterile environment to do tih!

[arvin555]

I totally agree with you guys that a sterile evironment would be ideal, but I am under the impression that one can get away with a "kitchen setting", there are some home buildable systems to create a sterile box too, web has some tips there.

I was in a gardening show a few months back and a local university had a display of their TC system (not CPs though) and they used a wooden box with a glass window and rubber gloves to create that environment, so even the universities are just doing "kitchen" style TC.


TTFN
Arvin

[edmund83]

ya... last time visit our Malaysia MAHA 2008. one of the local university did show and provide the course. It about RM799 include toolkit and course. the success is 50% chances.