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Old 8th September 2010, 12:32 PM
arvin555 arvin555 is offline
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Join Date: Tue Feb 2008
Location: Manila Philippines
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Default Re: in vitro first sprout

Robert congratulations! Did you see the info in kitchenTissueculture or hometissueculture about an additive that is supposed to help fight contaminants? It is called PPM and allows us to do TC at home, I have the kitchen culture kit but still unfortunately no time to dive into trying out TC myself So I'm happy that some of our brothers... like you are getting into it.

To others, please be informed that, though one can actually put additives that will allow for faster growth, the main purpose of TC is not really for faster growth but for faster propagation.

If Robert used 10 seeds and 1 germinated, it is not much use to grow this in TC and then plant it outside eventually. As some people mentioned you don't get the advantage, you have the same rate sowing them directly on potting medium.

But because that 1 plant that germinated is sterile, Robert can actually cut it up into several piece, add additive that will make the pieces sprout into new plants themselves.. so if you have 1 plant, you can get say 3 to 5, and the 3 to 5 plants can then be cut up and thus the multiplication factor increases! This is quite nice for rare plants. Which reminds me, Robert what species of seeds did you use? I mean don't get me wrong, once I get to try TC, I will try it out with not so rare seeds first.

Of course VFT is way easier, just get one leaf, disinfect it like crazy, and cut up and then put it in an additive and you should have a lot of VFTs soon. It's Nepenthes that is a big challenge.... good luck Robert!

Hopefully next time you can try out seeds of N. Attenboroughii

TTFN
Arvin
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