TC question

[Robert]

Originally Posted by winterkid

robert..
what kind of medium you use??
i saw that its a little blue and translucent..
mind sharing the recepie with me?
and where did you get the TC vessels?

the containers/vessels were bought from a local scientific co.

i made my own medium - sugar, coconut water, charcoal and agar.

[winterkid]

mind sharing the location or contact of the scientific co?

can share the exact perpotion of the ingredient for the recepi

[Robert]

Originally Posted by winterkid

mind sharing the location or contact of the scientific co?

can share the exact perpotion of the ingredient for the recepi

I'm in kuching and the co. imported the jars ( and other equipment and accessories ) were imported from, most likely, kuala lumpur.

To a liter coconut water ( or a liter distill water ) i add sugar 20 gm, charcoal 0.5gm, agar 8 gm ( the degree of softnes and watery-like to hardness of the medium depended on the amount of agar, you may play with the amount) 8gm is still soft. Too hard the seedlings roots can't penetrate the medium.

I read and gathered infos from the following link and try it out my own. you may like to prepare your own.
http://www.orchidboard.com/community...ng-medium.html

There is no right or wrong answer. some people add apple juice, banana, papaya, tomato. The ingredients are important to seedlings development else without food the seedlings turn brown and die.

[Harris Azariel]

whats charcoal for?

so you use the spirit metil(the once in bengkil) as for sterilization?

[Robert]

Originally Posted by Harris Azariel

whats charcoal for?

so you use the spirit metil(the once in bengkil) as for sterilization?

role of charcoal
http://www.ncbi.nlm.nih.gov/pubmed/18786626

for sterilisation i use ethanol, but preferable household bleach(clorox).

[Harris Azariel]

is ethanol rubbing alchohol?
hw much does it cost for hw much ethanol?

[Harris Azariel]

so u take the dust of the coal and measure till 0.5grams?


Thanks man!
Harris

[Robert]

Originally Posted by Harris Azariel

is ethanol rubbing alchohol?
hw much does it cost for hw much ethanol?

rubbing alcohol is methanol, contain ethanol but not as pure. yes, can be used to sterilised seeds and the working area ( inner sides of the glove hood and laminar floor)

ethanol was packed in 2.5 lit container.

You need to find the price in KL or anywhere in west malaysia, 'cos we are paying more for most item in the east.

[Robert]

Originally Posted by Harris Azariel

so u take the dust of the coal and measure till 0.5grams?


Thanks man!
Harris

i crush the charcoal then sieve and use the finer parts.

[Harris Azariel]

Rob,can u sure us the pic of the bottle of the ethanol?