in vitro first sprout

[Robert]

sharing with everyone my low tech set up and my first in vitro neps seeds germination. seeds sterilisation was using clorox and media was home made.

contamination rate was very high for me. There are several reason - from the glass hood ( fish aquarium ) construction method, sterilisation and handlings.



jars and containers were placed on window edge where it was bright and warm



still no germination



the first sprout among all the jar

[allenphoon]

can share the advantage using the vitro? i only know that it will grow much faster
BTW, i like this picture, look like a laboratory lab

[cbkhoon]

wow.. going to next level.. good luck on your new project !

[Harris Azariel]

where did u find Agar?

[rhealk]

Wow, nice setup!

[Robert]

yeah allen it was a mini lab. There are many reason if ones searched, as an example :
http://www2.ulg.ac.be/cedevit/english/advantage_e.htm

Originally Posted by cbkhoon

wow.. going to next level.. good luck on your new project !

hee..hee not that fast. I'm still a new hand in this field. Just curious and to try out the media preparation and sterilization method.

Harris the agar² can be bought from any grocery stores or where those specialised in selling ingredients for cakes making.

Thanks rhealk

[Harris Azariel]

thanks robert.do pm ME hw u sterilize the media and hw u prepare or make the media and hw do u sterilize the seeds.i just won some seeds from the flytrapcare.com.so i wanna try them in vitro.i just need to learn the preparation of media(sterilizng too),and deflasking it

[Robert]

Originally Posted by Harris Azariel

thanks robert.do pm ME hw u sterilize the media and hw u prepare or make the media and hw do u sterilize the seeds.i just won some seeds from the flytrapcare.com.so i wanna try them in vitro.i just need to learn the preparation of media(sterilizng too),and deflasking it

Harris, it's fine to answer yor question here since i searched all the info online. There are many, read through, pick up the details and note down.

Media preparation and sterilising procedure were similar to orchid seeds.

http://www.youtube.com/watch?v=TYz77RXs9Ho

http://www.flytrapcare.com/tissue-culture-basics.html

http://www.phytotechlab.com/Technica...e%20Techniques

Instead of using autoclave i use home pressure cooker to cook the media for 20 minutes ( take the reading once the pressure built up ). Dont use/share the pr.cooker for home food preparation. Get a new one. There are many brand to choose. A China made cost approx RM130.00 which, like most for home use, will achieve 15 psi. Any less cooking have to be adjusted.

Cooked media in jar - leave them for 7 days or longer to make sure no mold form.

If still have doubt please post here so every member can discuss.

[rhealk]

Wow, great effort, Robert. Perhaps, someday later you can market your 'sealed-pre-repared-in-vitro-jar' for commoners like me who want to try in vitro but do not have the expertise and equipments (and patients!).
Thank you for sharing.

[shawnintland]

Hi Robert. Congratulations! Hey, you (and others) might want to check out;
http://www.hometissueculture.org
A friend of mine in the US just took one of their workshops and was impressed. I've seen the website for years but never got into it myself. Carol, who started it is great and Frank (president) has a whole series of Nep TC videos on you-tube as well. The also sell/ship supplies of all sorts.

Best of luck with the rest!
~Shawn