in vitro first sprout

[Harris Azariel]

thanks robert.do pm ME hw u sterilize the media and hw u prepare or make the media and hw do u sterilize the seeds.i just won some seeds from the flytrapcare.com.so i wanna try them in vitro.i just need to learn the preparation of media(sterilizng too),and deflasking it

[Robert]

Originally Posted by Harris Azariel

thanks robert.do pm ME hw u sterilize the media and hw u prepare or make the media and hw do u sterilize the seeds.i just won some seeds from the flytrapcare.com.so i wanna try them in vitro.i just need to learn the preparation of media(sterilizng too),and deflasking it

Harris, it's fine to answer yor question here since i searched all the info online. There are many, read through, pick up the details and note down.

Media preparation and sterilising procedure were similar to orchid seeds.

http://www.youtube.com/watch?v=TYz77RXs9Ho

http://www.flytrapcare.com/tissue-culture-basics.html

http://www.phytotechlab.com/Technica...e%20Techniques

Instead of using autoclave i use home pressure cooker to cook the media for 20 minutes ( take the reading once the pressure built up ). Dont use/share the pr.cooker for home food preparation. Get a new one. There are many brand to choose. A China made cost approx RM130.00 which, like most for home use, will achieve 15 psi. Any less cooking have to be adjusted.

Cooked media in jar - leave them for 7 days or longer to make sure no mold form.

If still have doubt please post here so every member can discuss.

[rhealk]

Wow, great effort, Robert. Perhaps, someday later you can market your 'sealed-pre-repared-in-vitro-jar' for commoners like me who want to try in vitro but do not have the expertise and equipments (and patients!).
Thank you for sharing.

[shawnintland]

Hi Robert. Congratulations! Hey, you (and others) might want to check out;
http://www.hometissueculture.org
A friend of mine in the US just took one of their workshops and was impressed. I've seen the website for years but never got into it myself. Carol, who started it is great and Frank (president) has a whole series of Nep TC videos on you-tube as well. The also sell/ship supplies of all sorts.

Best of luck with the rest!
~Shawn

[Harris Azariel]

Thanks guys!if i go on TC,i will inform.

[Robert]

Thanks Shawn. I was in hometissueculture.org and mostly gathering for info

[Harris Azariel]

Robert,where did u find the MS vitamins,etc etc?

[Robert]

Originally Posted by Harris Azariel

Robert,where did u find the MS vitamins,etc etc?

most can be bought from scientific co. There should be plenty around your area.

[paphioboy]

Nice... But I don't think its worth doing in-vitro germination of neps, considering that the natural germination rate on moss is quite high anyway...

[Harris Azariel]

but it get matures fast!