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Old 14th September 2010, 12:48 AM
arvin555 arvin555 is offline
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Join Date: Tue Feb 2008
Location: Manila Philippines
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Default Re: in vitro first sprout

Robert, Aww oh come on! Try Meristem! Cut a piece of nepenthe stem, then sterilize... Then cut off a few layers and then sterilize, until you get to the middle part of the stem (meristem) then sterilize and then TC I heard that they said that the hard part is to get a sterile sample... I don't know if that is accurate, I didn't hear anyone say that the tissue does not work, it's that usually you usually find that the Tissue gets contaminated. But as I said that is based on what I heard or read. I might have had some discussion about it here somewhere.

In any case for nepenthes, the norm is to TC the plants that sprout from seeds, at least those are sterile.

TTFN
Arvin
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