Tissue Culture Thread

[nepaholic]

Hi

just want to show some jars from me.

Here a VFT from Seed (5month old)


VFT Red form. TC from Leaf cutting, Now develop their first leaves after i replant this to plain media.


VFT B52 make now callus. i just transferred it to plain media and hope there come the first leaves soon


here some other VFT from seeds




Jens

[kentosaurs]

Hi Nepaholic

Nice VFts I like them.........You grew the seeds inside the syrup thingy????????????

Ken

[plantlover]

Nice VFTs. How long did it take for them to germinate??

[nepaholic]

Thank you

it takes 4-6 weeks for germination in TC.
But then they grow very fast.
The last 2 seedlings i sow in august. So they germinate in September.

You can see how fast they grow, every day

Jens

[arvin555]

may we know what TC medium you use and what preparations you made in terms of chemicals, etc. on your seed and on your leaf cutting? Did you use hormones, etc?

I'm hoping that I can finally buy a TC kit and start a project similar to yours.

Oh and where do you keep the bottles? What lighting?

TTFN
Arvin

[nepaholic]

Hi Leafs are little bit tricky and i recommend to learn first TC with seeds.
I use 1/4 ms for seeds, for leafs just add 1ml/L Bap.
Seeds i sterilise 15min in 10%Bleach
Why you want to buy a kit?
You can build a simple transfer box with gloves. thats cheaper.

Jens