TC question

[winterkid]

i would like to have someone teach me more about tc ....
i just wan to know the mixture for their medium and where can i get the ingerdients....?
pls discribe the ingerdients more........because i may not understand.......
i saw the web and found
http://www.phytotechlab.com/Detail.aspx?ID=1016
its salt for neps but can it be used for vfts?
i really need step by step guide on TC medium
where do i get MS?????

[tzestan]

I have no experience in tc.
You can check with Quicklab in Batu Lanchang. I used to buy reagent grade chemical salts there. Mixing MS from component salts is tricky. Probably they have MS. I'll PM you their contact.

[Harris Azariel]

Tan,PM me their contact info also!!

thanks!!

[winterkid]

what about Tc vessels...and sterlizing equepments???? where to find?

[edwardyeeks]

I'll be honest with you guys, I've heard that TC is quite hard to do. Even setting up TC materials can be troublesome and kinda expensive. A few members here do it, but most don't because it requires a depth of knowledge to actually do TC. But it's no harm done if you guys are willing to try. Good luck!

[allenphoon]

From what i know, if you really willing to start TC, you will have to bear some problems

1)scolded by your mom or your wife(you need to use pressure cooker-to kill germ, etc)
2)pay lots of electrical bills( you will need artificial light instead of natural life as they are keep in vessel)
3)space(you will have to set up a completely no germ/microorganism condition for tc preparation/separation)
4)distilled water(just as the ingredient)

If you are a student, it is not advisable as it cost a lot and require long patience

[Robert]

How to do TC ...there are tons of info in the web. print out those you find useful and read them like the holy bookor jot down the important info.

The mixture - for a beginner to try out, there is no need to use expensive ingredients. Home made medium will do, the same preparation as orchid growers do.what is required, read the following link.

http://www.rv-orchidworks.com/orchid...opagation.html

To sterilise jars(with or without ingredients) and water ordinary pressure cooker is required.

jam jars, beer mugs are excellent. ordinary plastic containers can be used but need to be sterilised before pouring in the cooked ingredients. This has to be done in a sterile chamber ( a laminar flow or glove hood)

different jars that i use



germination in-vitro. nepenthes are difficult and near impossible to TC from tips, stems, roots or leaves. start with seeds first.





...and i use artificial light placing the jars on window sills, lights are filtered.


MS and other mixtures,equipments and accessories can be bought from most scientific co.

I use fish aquarium as sterile hood.
https://forum.petpitcher.net/showthread.php?t=4471

Originally Posted by winterkid

i would like to have someone teach me more about tc ....
i just wan to know the mixture for their medium and where can i get the ingerdients....?
pls discribe the ingerdients more........because i may not understand.......
i saw the web and found
http://www.phytotechlab.com/Detail.aspx?ID=1016
its salt for neps but can it be used for vfts?
i really need step by step guide on TC medium
where do i get MS?????

[edwardyeeks]

Wow Robert, that's very nice and complex! How long did it take for the seeds to germinate?

[Robert]

Originally Posted by edwardyeeks

Wow Robert, that's very nice and complex! How long did it take for the seeds to germinate?

22 days.

[winterkid]

for the sterlie hood......how do you put or remove the TC from it without contaminating them?? mind showing pics or state steps??