TC question

[Robert]

How to do TC ...there are tons of info in the web. print out those you find useful and read them like the holy bookor jot down the important info.

The mixture - for a beginner to try out, there is no need to use expensive ingredients. Home made medium will do, the same preparation as orchid growers do.what is required, read the following link.

http://www.rv-orchidworks.com/orchid...opagation.html

To sterilise jars(with or without ingredients) and water ordinary pressure cooker is required.

jam jars, beer mugs are excellent. ordinary plastic containers can be used but need to be sterilised before pouring in the cooked ingredients. This has to be done in a sterile chamber ( a laminar flow or glove hood)

different jars that i use



germination in-vitro. nepenthes are difficult and near impossible to TC from tips, stems, roots or leaves. start with seeds first.





...and i use artificial light placing the jars on window sills, lights are filtered.


MS and other mixtures,equipments and accessories can be bought from most scientific co.

I use fish aquarium as sterile hood.
https://forum.petpitcher.net/showthread.php?t=4471

Originally Posted by winterkid

i would like to have someone teach me more about tc ....
i just wan to know the mixture for their medium and where can i get the ingerdients....?
pls discribe the ingerdients more........because i may not understand.......
i saw the web and found
http://www.phytotechlab.com/Detail.aspx?ID=1016
its salt for neps but can it be used for vfts?
i really need step by step guide on TC medium
where do i get MS?????

[edwardyeeks]

Wow Robert, that's very nice and complex! How long did it take for the seeds to germinate?

[Harris Azariel]

Thanks Robert!

For me now,keeping the media sterile when its out from the microwave is hard since that i cant tight the cap and most of my media contaminated at that part.

[Robert]

Originally Posted by edwardyeeks

Wow Robert, that's very nice and complex! How long did it take for the seeds to germinate?

22 days.

[winterkid]

for the sterlie hood......how do you put or remove the TC from it without contaminating them?? mind showing pics or state steps??

[Harris Azariel]

heres how,
http://www.youtube.com/watch?v=wJeEDKYfeoA

Frank only use normal open wide hoods but he had lots of succesfull TC vessels.

[winterkid]

i found this web page...
and its going to open in malaysia....
http://www.caissonlabs.com/services.php?group=2

woopie

[Robert]

Originally Posted by winterkid

for the sterlie hood......how do you put or remove the TC from it without contaminating them?? mind showing pics or state steps??

before carrying out work the inside hood have to be sterilised using bleach or spirit. The jars with the ingredients are cooked withr pressure cooker for 25 minutes and they are sterile. They are opened only inside the sterilised hood ( do not open outside as that will let contaminants in )

[Robert]

Originally Posted by winterkid

i found this web page...
and its going to open in malaysia....
http://www.caissonlabs.com/services.php?group=2

woopie

hmmm, the polycarbonate vessels look exactly i'm using

[winterkid]

robert..
what kind of medium you use??
i saw that its a little blue and translucent..
mind sharing the recepie with me?
and where did you get the TC vessels?