in vitro first sprout

[Robert]

Originally Posted by Harris Azariel

thanks robert.do pm ME hw u sterilize the media and hw u prepare or make the media and hw do u sterilize the seeds.i just won some seeds from the flytrapcare.com.so i wanna try them in vitro.i just need to learn the preparation of media(sterilizng too),and deflasking it

Harris, it's fine to answer yor question here since i searched all the info online. There are many, read through, pick up the details and note down.

Media preparation and sterilising procedure were similar to orchid seeds.

http://www.youtube.com/watch?v=TYz77RXs9Ho

http://www.flytrapcare.com/tissue-culture-basics.html

http://www.phytotechlab.com/Technica...e%20Techniques

Instead of using autoclave i use home pressure cooker to cook the media for 20 minutes ( take the reading once the pressure built up ). Dont use/share the pr.cooker for home food preparation. Get a new one. There are many brand to choose. A China made cost approx RM130.00 which, like most for home use, will achieve 15 psi. Any less cooking have to be adjusted.

Cooked media in jar - leave them for 7 days or longer to make sure no mold form.

If still have doubt please post here so every member can discuss.