in vitro first sprout

[Harris Azariel]

u can make them root fast by putting a few of the rooting hormone that u can obtain at local store.not just fast,but the roots will be crowded with each to other strands

[Robert]

Originally Posted by arvin555

Robert congratulations! Did you see the info in kitchenTissueculture or hometissueculture about an additive that is supposed to help fight contaminants? It is called PPM and allows us to do TC at home, I have the kitchen culture kit but still unfortunately no time to dive into trying out TC myself So I'm happy that some of our brothers... like you are getting into it.



TTFN
Arvin

Hi Arvin, i've read in most post ppm was highly recommended. I have problem getting most chemical locally. Suppliers are not keen for small purchase. In most cases it had to be flown from the peninsular, and costly. Thus i use clorox and spirit which turn out as good, and inexpensive. The only problem was the wide margin of error. contamination,i read , can happen due to the age of spirit and clorox. using these to sterilise seeds ones have to adjust the time to sterilise seeds.

Originally Posted by Harris Azariel

hey Robert,wanna try VFT seeds on TC?

thanks Harris, if you have some to spare

Originally Posted by allenphoon

Robert, sorry for bothering, but would you mind PM me the information as well? i am getting interested in TC.....
and may i know so far how much had you spent in this? i mean from TC until the plant can transplant into normal media...thankyou

the detail from my pmed, you need to call or email them for the items you required.

[lcuclcu]

Originally Posted by Robert

sharing with everyone my low tech set up and my first in vitro neps seeds germination. seeds sterilisation was using clorox and media was home made.

contamination rate was very high for me. There are several reason - from the glass hood ( fish aquarium ) construction method, sterilisation and handlings.



jars and containers were placed on window edge where it was bright and warm



still no germination



the first sprout among all the jar

a great project....your tools are very nice

[allenphoon]

if i really can spare vft seeds, will you try?

[arvin555]

Hi Robert,

From what I understand, PPM is an additive to the Agar itself, and prevents contamination on the medium... contamination of your Tissue is of course through sterilization such as your mentioned Chlorine, some actually say Hydrogen Peroxide, etc. I also think that once the Tissue is in the TC medium, the PPM will also probably protect it from Contamination as well.

I have a feeling that Kitchen Tissue Culture will be able to ship directly to you, if you order from them. That would already be as simple or as complicated as buying from Peninsular Malaysia.

Just my 2 cents here, I think that because VFT leaves are easy to disinfect... no need to start from seeds, you can just cut a leaf or two and that is the start of your Tissue, just disinfect the leaves or pieces properly. At least this way, no waiting for germination. As mentioned the only CP that is difficult to TC are Neps, because apparently getting Tissues from nepenthes plants and successfully and effectively sterilizing them is almost impossible.

So if you don't mind, and have the time.... May I challenge you or request you to please experiment by cutting up one of your more common nepenthes plant (just a stem cutting don't worry) and try to get a Meristem from it and then try to get that going in TC medium.

TTFN
Arvin

[Robert]

Arvin, i've read from many who had tried, and agreed neps was impossible to TC using their stems. Infact non of their shoot tip, leaf, buds, stem or root tissue can be TC.

[Robert]

Originally Posted by allenphoon

if i really can spare vft seeds, will you try?

Thanks allen someone had sent me sarras and vft seeds.

[Harris Azariel]

if u have success on VFTs and Sarrs,u can sell them!

[arvin555]

Robert, Aww oh come on! Try Meristem! Cut a piece of nepenthe stem, then sterilize... Then cut off a few layers and then sterilize, until you get to the middle part of the stem (meristem) then sterilize and then TC I heard that they said that the hard part is to get a sterile sample... I don't know if that is accurate, I didn't hear anyone say that the tissue does not work, it's that usually you usually find that the Tissue gets contaminated. But as I said that is based on what I heard or read. I might have had some discussion about it here somewhere.

In any case for nepenthes, the norm is to TC the plants that sprout from seeds, at least those are sterile.

TTFN
Arvin

[Robert]

Originally Posted by arvin555

Robert, Aww oh come on! Try Meristem! Cut a piece of nepenthe stem, then sterilize... Then cut off a few layers and then sterilize, until you get to the middle part of the stem (meristem) then sterilize and then TC I heard that they said that the hard part is to get a sterile sample... I don't know if that is accurate, I didn't hear anyone say that the tissue does not work, it's that usually you usually find that the Tissue gets contaminated. But as I said that is based on what I heard or read. I might have had some discussion about it here somewhere.

In any case for nepenthes, the norm is to TC the plants that sprout from seeds, at least those are sterile.

TTFN
Arvin

maybe one day when i've developed the skilled with all the sophisticated chemicals available i would give a try.